F-410 DyNAmo HS SYBR organic master mix (Thermo Healthcare, MA, USA) using QuantStudio 12 K flex real-time PCR system (Invitrogen, CA, USA). Beta actin was used for normalizing the gene overall look stages. Primer sequences used to quantify endogenous gene overall look stages are listed in Desk S1 in Computer data file S1. Isolation and Decellularization of Amniotic Membrane Amniotic cells part was divided from the placenta illustrations. Amniotic cells part was washed with PBS thoroughly before proceeding towards decellularization. The decellularization of the amniotic cells part was taken out as described formerly by Stacy Paul et al., [26]. Briefly, the cells part was initially incubated with hypotonic 10 mM Tris buffer containing 0.1% EDTA and 10 kiu/mL aprotonin for overnight.
