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Fig. SM4. Diagram for explaining four radioactive color LC three points, shown in Fig. SM2. (Bárány and Bárány, 1996a, with permission Alpha Limit  of Biochemistry, smooth muscle contraction, 1996, Academic Press).

The last row of Fig. SM2 shows muscle-contraction induced changes in the intensity of staining of LC spots. In resting muscle the last peak, the major isoform phosphorylated contains as much as 63% of the total intensity, which decreases to 35% in hiring, muscle. At the same time, the intensity of 23% in the previous peak of resting muscle increases to 49% shrinkage. This is an example of a simple method (but not sensitive) to track changes in the phosphorylation of LC during contraction without using 32P-labeled muscle.


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